type 19 rotor (Danaher Inc)

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Danaher Inc type 19 rotor
Type 19 Rotor, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/type+19+rotor/Type+19+Rotor+Package/pm40540783-66-17-25
Average 93 stars, based on 131 article reviews

type 19 rotor - by Bioz Stars, 2026-10

93/100 stars

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Centrifugation:

Article Title: Kaposi’s Sarcoma-Associated Herpesvirus-Encoded circRNAs Are Expressed in Infected Tumor Tissues and Are Incorporated into Virions
Article Snippet: Briefly, to induce the KSHV lytic cycle, BJAB-rKSHV.219 cells were inoculated at a density of 6 × 10 5 cells/ml in a total volume of 800 ml culture in the presence of 3 μg/ml of anti-human IgM antibody (catalog no. 2020–01; SouthernBiotech) and grown for 4 to 5 days in Techne spinner flasks (Cole-Parmer GmbH, Wertheim, Germany) with 60 rpm agitation. .. Culture supernatant was collected after low-speed centrifugation was performed to remove cells and debris and was concentrated by ultracentrifugation at 15,000 rpm for 5 h in a type 19 rotor (Beckman Coulter Inc., Brea, CA, USA). .. The pelleted virus was resuspended in 1× phosphate-buffered saline (PBS) overnight, layered onto a 20% to 35% Histodenz (D2158; Sigma) gradient, and centrifuged at 71,000 × g for 2 h using an SW41Ti rotor (Beckman).

Article Title: Kaposi’s Sarcoma-Associated Herpesvirus-Encoded circRNAs Are Expressed in Infected Tumor Tissues and Are Incorporated into Virions
Article Snippet: Briefly, to induce the KSHV lytic cycle, BJAB-rKSHV.219 cells were inoculated at a density of 6 105 cells/ml in a total volume of 800 ml culture in the presence of 3 g/ml of anti-human IgM antibody (catalog no. 2020 – 01; SouthernBiotech) and grown for 4 to 5 days in Techne spinner flasks (Cole-Parmer GmbH, Wertheim, Germany) with 60 rpm agitation. .. Culture supernatant was collected after low-speed centrifugation was performed to remove cells and debris and was concentrated by ultracentrifugation at 15,000 rpm for 5 h in a type 19 rotor (Beckman Coulter Inc., Brea, CA, USA). .. The pelleted virus was resuspended in 1 phosphate-buffered saline (PBS) overnight, layered onto a 20% to 35% Histodenz (D2158; Sigma) gradient, and centrifuged at 71,000 g for 2 h using an SW41Ti rotor (Beckman).

Recombinant:

Article Title: Chimeric designs of rabies virus glycoprotein G enhance baculovirus pseudotyping and immunogenicity in mice: influence of the transmembrane domain and a flexible linker.
Article Snippet: .. Supernatants containing each recombinant baculovirus were ultracentrifuged at 48,630×g for 15 h at 4 ◦C using a Type 19 rotor in an Optima XPN-100 ultracentrifuge (Beckman Coulter, Brea, CA, USA). ..

Sedimentation:

Article Title: The interferon-inducible GTPase MxB promotes capsid disassembly and genome release of herpesviruses
Article Snippet: .. Extracellular HSV-1 or HSV-2 particles were harvested by sedimentation at 12,000 rpm for 90 min at 4 °C (Type 19 rotor, Beckman-Coulter) from the medium of BHK-21 cells (40 × 175 cm2 flasks; 2–2.5 x 10 7 cells/flask) infected with 0.01 PFU/cell (2–6.7 x 10 4 PFU/mL) for 2.5 days. .. The resulting medium pellets (MP) were resuspended in 2 mL of MKT buffer (20 mM MES, 30 mM Tris-HCl, 100 mM KCl, pH 7.4), treated with 0.5 mg/mL trypsin (Sigma-Aldrich, Germany) at 37 °C for 1 hr which was then inactivated with 5 mg/mL trypsin inhibitor from soybean (SBTI; Fluka, Switzerland) for 10 min on ice ( ; ; ; .

Article Title: The interferon-inducible GTPase MxB promotes capsid disassembly and genome release of herpesviruses
Article Snippet: .. Extracellular HSV-1 or HSV-2 particles were harvested by sedimentation at 12,000 rpm for 90 minutes at 4°C (Type 19 rotor, Beckman-Coulter) from the medium of BHK-21 cells (40 x 175 cm2 flasks; 2 – 2.5 x 10 7 cells/flask) infected with 0.01 PFU/cell (2 to 6.7 x 10 4 PFU/mL) for 2.5 days. .. The resulting medium pellets (MP) were resuspended in 2 mL of MKT buffer (20 mM MES, 30 mM Tris-HCl, 100 mM KCl, pH 7.4; ; ; ), treated with 0.5 mg/mL trypsin (Sigma-Aldrich, Germany) at 37°C for 1 h which was then inactivated with 5 mg/mL trypsin inhibitor from soybean (SBTI; Fluka, Switzerland) for 10 min on ice.

Infection:

Article Title: The interferon-inducible GTPase MxB promotes capsid disassembly and genome release of herpesviruses
Article Snippet: .. Extracellular HSV-1 or HSV-2 particles were harvested by sedimentation at 12,000 rpm for 90 minutes at 4°C (Type 19 rotor, Beckman-Coulter) from the medium of BHK-21 cells (40 x 175 cm2 flasks; 2 – 2.5 x 10 7 cells/flask) infected with 0.01 PFU/cell (2 to 6.7 x 10 4 PFU/mL) for 2.5 days. .. The resulting medium pellets (MP) were resuspended in 2 mL of MKT buffer (20 mM MES, 30 mM Tris-HCl, 100 mM KCl, pH 7.4; ; ; ), treated with 0.5 mg/mL trypsin (Sigma-Aldrich, Germany) at 37°C for 1 h which was then inactivated with 5 mg/mL trypsin inhibitor from soybean (SBTI; Fluka, Switzerland) for 10 min on ice.

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